Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: May 5 2011
Last update date: Aug 10 2018
Access: Public
Diseases: Hodgkin Disease, Multiple Myeloma, Lymphoma, B-Cell
Dataset link Classical Hodgkin lymphoma shows epigenetic features of an abortive plasma cellular differentiation: expression of Hodgkin vs. B-cell lines

Experimental Protocol


Gene expression analysis of Hodgkin lymphoma (cHL) and B-cell lines: Microarray data for three Hodgkin lymphoma cell lines (KM-H2, L1236, L428) and the B-cell line Namalwa that were published previously by our group (GEO accession GSE8388) were analyzed together with newly generated data for the B-cell lines SU-DHL4 and SU-DHL6. For all cell lines, RNA was isolated according to standard protocols (Qiagen, Hilden, Germany) and used for Affymetrix GeneChip hybridization (HG-U133A). Microarrays were normalized using RMA, and differential expression was calculated using moderated t-test. The gene expression profiles of the cell lines were generated in duplicates.

Repositories


GEO

GSE21251

ArrayExpress

E-GEOD-21251

BioProject

PRJNA129721

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Contact


Volkhard Seitz