Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 66
Release date: Dec 3 2016
Last update date: Nov 30 2018
Access: Public
Diseases: Hypothalamic Neoplasms
Computational protocol: TopHat, Bowtie2, Cufflinks
Dataset link A neural basis for melanocortin-4 receptor-regulated appetite.

Experimental Protocol


Single-neuron mRNA-seq was performed on fluorescently-labeled or -unlabeled cells that were manually isolated from dissociated adult mouse paraventricular and arcuate hypothalamus: Mc4r-2a-Cre::L10-GFP+ or Mc4r-2a-Cre::AAV-XFP+ or Mc4r-2a-Cre::AAV-XFP-negative PVH neurons; Agrp-IRES-Cre::L10-GFP+ ARC neurons; Pomc-hrGFP+ ARC neurons; and vGLUT2-IRES-Cre::AAV-XFP+ ARC neurons Note: Raw files unavailable for samples GSM2413312 GSM2413313 GSM2413314 GSM2413346 GSM2413347

Repositories


GEO

GSE90809

ENA

SRP094473

BioProject

PRJNA355997

Download


Contact


John Campbell

Dataset Statistics

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Dataset publication