|Dataset type:||Expression profiling by high throughput sequencing|
|Number of samples:||8|
|Release date:||Jan 29 2019|
|Last update date:||Feb 1 2019|
|Dataset link||Interspecies chemical signalling in a methane-oxidizing bacterial community|
In order to determine which genes MmsR regulates in response to acyl-HSL signal, we compared the transcriptome of exponentially growing LW13 in the presence or absence of 3-OH-C10-HSL. As a control for gene changes that are not the result of signal binding to MmsR, we constructed an unmarked, in-frame deletion of mmsR (∆mmsR) and also compared the transcriptome of this strain in the presence and absence of signal. For each condition, we sequenced the transcriptomes of two biological replicates.