|Dataset type:||Expression profiling by RT-PCR, Transcriptome or Gene expression|
|Number of samples:||36|
|Release date:||Oct 18 2014|
|Last update date:||Feb 29 2016|
|Diseases:||Chlamydia Infections, Fibrosis, Infertility, Inflammation|
|Dataset link||Differential expression of mRNA encoding cytokines and chemokines in the reproductive tract after infection of mice with Chlamydia trachomatis|
Six to eight week old female C3H/HeJ mice (Jackson Laboratories, Sacramento, CA) were pretreated with 2.5 mg medroxyprogesterone acetate (SICOR Pharmaceuticals) on Days -10 and -3 before infection. On Day 0, mice were infected via vaginal challenge with 0 or 5 x 102 inclusion-forming units (IFUs) of C. trachomatis, serovar D in 0.01 mL of Eagle Minimal essential media (MEM, Gibco). Mice were killed on day 28 (n = 3 for control and infected) in experiment 1 and day 35 (n = 3 for control and infected) in experiment 2. Vaginal tissue, uterine horns and the oviducts were collected and processed for superarray analysis.