Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 5
Release date: Oct 3 2012
Last update date: Aug 23 2018
Access: Public
Chemicals: Glucose
Mutations: p|SUB|P|467|L, p|SUB|V|290|M
Dataset link Gene expression profiling during interference with PPAR gamma signaling in thoracic aorta

Experimental Protocol


We generated transgenic mice specifically targeting expression of mutant dominant negative human PPAR gamma (P467L) to vascular smooth muscle using a smooth muscle-specific promoter (smooth muscle myosin heavy chain or SMMHC). Thoracic aortas were isolated from male transgenic mice and corresponding non-transgenic littermate controls. Total RNA was prepared using conventional methods and quality was assessed using the Bioanalyzer 2100 (Agilent Technologies). For the microarray hybridizations, 2 samples from control mice and 3 samples from transgenic mice were used. Each sample was an independent biological replicate generated by pooling total RNA from 6-8 separate mouse aortas. All procedures were conducted at the University of Iowa DNA Core facility using standard Affymetrix protocols. In brief, approximately 3-5 ug of total RNA was used as input to a one-step amplification procedure to generate biotin-labeled RNA fragments for hybridization to the Affymetrix Mouse Genome 430 2.0 array.

Repositories


GEO

GSE37194

ArrayExpress

E-GEOD-37194

BioProject

PRJNA158653

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Contact


Henry Keen