Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 7
Release date: Oct 20 2013
Last update date: Nov 1 2017
Access: Public
Genes: GFI1, IL5, IL13
Dataset link Mouse ILC2 cells from wild-type or Gfi1-KO animals

Experimental Protocol


Gfi1+/+ or Gfi1GFP/GFP (a GFP cassette has been 'knocked-in' to the Gfi1 locus and thus functions both as a surrogate for Gfi1 promoter activity and as a loss-of-function allele) mice were injected with IL-25 once daily for 4 days. On day 5, ILC2s (Lin-/Sca-1+/ICOS+/CD127+/c-Kit+) were sorted from the mesenteric lymph nodes of Gfi1+/+ (3 mice/sample were pooled together) or Gfi1-/- mice (6 mice/sample were pooled together) and cultured for 6 days in RPMI 1640, 10% FBS, Gln, P/S, 2ME, IL-2 (50ng/ml), IL-7 (10ng/ml), and IL-25 (50ng/ml). Whole RNA was harvested on day 6 via the RNeasy kit (Qiagen).

Repositories


GEO

GSE45621

ArrayExpress

E-GEOD-45621

BioProject

PRJNA195417

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Contact


Alex Abbas