Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Feb 8 2012
Last update date: Nov 1 2017
Access: Public
Diseases: Agammaglobulinemia, Leukemia, Myelogenous, Chronic, BCR-ABL Positive, Neoplasms, Second Primary
Chemicals: Cytidine
Dataset link Gene expression profile induced by Imatinib

Experimental Protocol


We investigated that class switch recombination(CSR) upon B cell activation is apparently inhibited by imatinib mesylate(IM) through downregulation of activation-induced cytidine deaminase(AID). Furthermore, expression of E2A was markedly suppressed by IM. To elucidate the more detailed pathway, we performed the microarray analysis. Microarray analysis was performed on splenocytes cultured for 72 h in the presence or absence of 10 µM Imatinib. The mouse splenocytes were cultured for 72 h with or without 10 µM Imatinib (IM) in conditioning medium containing IL-4 and LPS. RNA from 1X10^6 splenocytes used for microarray analysis was isolated using the RNeasy Mini Kit (50) (Qiagen, Hilden, Germany). Gene expression microarray analysis was performed using one-color microarray-based gene-expression analysis (Agilent Technologies, Santa Clara, CA, USA) according to the manufacturer’s instructions. After scanning, expression values for the genes were determined using GeneSpringGX software.

Repositories


GEO

GSE35559

ArrayExpress

E-GEOD-35559

BioProject

PRJNA152679

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toyotaka kawamata