Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Jan 10 2013
Last update date: Apr 11 2013
Access: Public
Diseases: Neoplasms, Leukemia, Promyelocytic, Acute
Computational protocol: lumi, limma, Dynamic Tree Cut, GeneAnswers, MACAT
Dataset link Interaction effects of knockdown of PML and TNFalpha treatment in HUVEC cells

Experimental Protocol


The experiment is a 3x2 two-factor design. One factor is siRNA and it has three levels (siCtrl, siPML1, siPML2). siCtrl is RISC-inducing non-targeting control siRNA. siPML1 and siPML2 designate two independent siRNAs against the PML gene. Two independent siRNAs were used to eliminate the off-target effects of siRNA. The other factor is TNFalpha treatment (20 ng/mL for 20 h) and it has two levels: Untreated (U) or Treated (T). Each sample has technical duplicates.

Repositories


GEO

GSE35000

ArrayExpress

E-GEOD-35000

BioProject

PRJNA151067

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Contact


Hung-Ying Kao

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