|Application:||Gene expression microarray analysis|
|Number of samples:||48|
|Release date:||Jan 23 2018|
|Last update date:||Jan 23 2018|
|Diseases:||Diabetes Mellitus, Type 1, Diabetic Neuropathies|
|Dataset link||Modulation of the gastrointestinal microbiota normalizes the systemic inflammation and islet immunocyte recruitment capacity associated with autoimmune diabetes (in Biobreeding rats) [RatIslet_Longitudinal]|
In order to investigate T1D susceptibility at the target tissue level, we longitudinally examined the islet transcriptomes of using RNA pools of DRlyp/lyp, DR+/+, Flyp/lyp, and F+/+ rats provided normal cereal chow (n=6-8 rats per pool, technical triplicates were conducted at experimental condition). The study design encompassed day 20 (weaning), day 30 (prior to histological detection of Ccl11 expression by BB rat β-cells), day 40 (prior to insulitis in DRlyp/lyp rats), and day 50 (after insulitis but prior to diabetes onset in DRlyp/lyp rats). Unsupervised PCA found the islet transcriptomes of young and old rats were highly distinct, and the longitudinal data set for each stain was analyzed with STEM (Short Time Series Expression Miner).