Computational protocol: Role of sfk1 Gene in the Filamentous Fungus Penicillium roqueforti

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Protocol publication

[…] The deduced sequence of Sfk1 protein from P. roqueforti CECT 2905 was used to find similar sequences using BlastP. Sequences obtained were used to perform phylogenetic analysis. Briefly, sequences were aligned by Clustal Omega and the phylogenetic trees were reconstructed using the Neighbor-joining method with the MEGA version 7 program (). Evolutionary distances data were calculated using the Poisson correction model. The support of internal nodes was performed by bootstrap analyses with 1,000 replications. […]

Pipeline specifications

Software tools BLASTP, Clustal Omega
Application Phylogenetics
Organisms Penicillium roqueforti, Saccharomyces cerevisiae
Chemicals Mycophenolic Acid