Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Mar 23 2012
Last update date: May 3 2012
Access: Public
Chemicals: Cholesterol Esters
Genes: CR3, ATF3
Dataset link Transcriptional study of oxLDL-induced foam cell formation in WT and Atf3-/- murine bone marrow macrophages

Experimental Protocol


Twelve female mice (six Atf3-/-, and six WT control mice) were sacrificed at 8-12 weeks of age, and macrophages were derived from the femoral bone marrow using rhM-CSF. oxLDL was introduced into the medium for six samples (3 WT and 3 Atf3-/-) at 25 ug/mL on day seven, and after 24 h of incubation, RNA was isolated using Trizol. Labeled cRNA derived from the RNA samples was hybridized to Affymetrix Mouse Exon Array 1.0 ST GeneChips. Microarray data were processed using transcript-level probesets, and are in log2 scale.

Repositories


GEO

GSE32358

ArrayExpress

E-GEOD-32358

BioProject

PRJNA147231

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Contact


Alan Aderem

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