Computational protocol: Multilocus Sequence Typing Analysis of Carbapenem-Resistant Acinetobacter baumannii in a Chinese Burns Institute

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Protocol publication

[…] eBURST version 3.0 was used to analyze the MLST data to determine the evolutionary relationships among the isolates (; ). All the reported MLST data were downloaded from the PubMLST database. The default definition (sharing six of seven total alleles) was used to find the CC for the groups. Clustal X2 was used for the multi-sequence alignments and the alignment results were also used to construct a phylogenetic tree using the maximum parsimony method within PhyML(). […]

Pipeline specifications

Software tools BURST, Clustal W, PhyML
Databases PubMLST
Applications Phylogenetics, WGS analysis