Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 19
Release date: Mar 5 2018
Last update date: Nov 30 2018
Access: Public
Dataset link Bcl11b- and GATA3-mediated gene regulation in Th2 cells

Experimental Protocol


Naïve CD4 T cells were isolated from Bcl11bfl/fl-CreERT2 mice and cultured under Th2 conditions with or without 4-OHT for 4 days (early Th2 cells). Naïve CD4 T cells were isolated from Bcl11bfl/fl-CreERT2 mice and cultured under Th2 conditions for 4 days. Then, after resting in IL-2-containing medium for one day, the primed cells were further cultured under Th2 conditions with or without 4-OHT for 4 days (late Th2 cells). ChIP-Seq of Bcl11b and GATA3 analysis in early and late WT or KO Th2 cells. RNA-seq for early and late WT or KO Th2 cells, with 2 replicates. Raw data is in SRP104190.

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Andrew Oler