Dataset features


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Aug 25 2017
Last update date: Aug 25 2017
Access: Public
Taxon: Danio rerio
Dataset link The Increased Toxicity of UV-Degraded Nitroguanidine and IMX-101 Exposures in Zebrafish Larvae: Evidence Implicating Oxidative Stress [5]

Experimental Protocol

Six separate microarray analyses were conducted within this study. The 6 designs were conduced as follows: 1. NQ parent compound, 2. UV-Treated NQ, 3. Two-way design, NQ Parent x UV-Treated NQ, 4. IMX-101 parent material, 5. UV-treated IMX-101, 6. Two way design, IMX-101 parent x UV-Treated IMX-101. This GEO entry represents analysis number 5. - UV-treated IMX-101: Zebrafish larvae were exposed to IMX-101 that had been irradiated with UV-light where initial measured concentrations of the exposures included: 0 (control),1.9, 3.8, 7.6, 15.2, 30.2, 85.2, and 121.9 mg/L (measured concentrations). The UV-treatment was performed by exposing the prepared stock solutions of IMX-101 in a photo-reactor for 4 h. This duration of UV-treatment correlated to the UV-content of 48 h of sunlight in Vicksburg, Mississippi, USA at 450 lux (UV range only). Briefly, a fan cooled Rayonet Reactor RPR-100 (Southern New England Ultraviolet Company, CT) equipped with a carousel and sixteen 14 W black lamps (SNE Ultraviolet Company k = 300 ± 50 nm) to create a photo-intensity of 0.05 mW cm-2nm-1 was used for all UV-degradation experiments. These conditions were selected in accordance with EPA standard method 712-C-08-13 and represent the most active portion of light associated with photo-degradation. All bioassays using the UV-treated IMs were initiated within 3 hours of completion of the UV-treatment, where standard exposure methods were conducted free of UV-light. Exposure and control water was dechlorinated tap water (Vicksburg, MS USA municipal dechlorinated via activated carbon filtration) amended with artificial sea salts (Instant Ocean, Blacksburg, VA, USA) to a conductivity of 600 µS/cm. Danio rerio larvae were exposed in static nonrenewal acute 96-h bioassays. Exposure chambers were 300-ml high-form lipless beakers with a test solution volume of 250 ml. Ten larval fish per beaker were exposed to IMX-101 where all treatments included 4 exposure replicates. Larval zebrafish were fed newly hatched Artemia nauplii two hours before the initiation of exposures and at the 48-h timepoint. Surviving fish were enumerated daily and any fish found to be deceased were promptly removed from exposure chambers.








Kurt Gust