Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: May 26 2009
Last update date: Aug 28 2018
Access: Public
Diseases: Breast Neoplasms, Neoplasms
Dataset link Expression analysis in a breast cancer metastasis model

Experimental Protocol


For each cell line, 3 biological replicates were grown, RNA isolated, and gene expression profiles determined using Affymetrix HGU133_Plus_2 genechips. The MDA-MB-468GFP-LN cell line was the "experimental" group, and the MDA-MB-468GFP was the control group. Intensity values were calculated using MAS5.0, Data was then imported into Genespring 7.0, and ANOVA (nominal alpha set to 0.05) was used to determine probe sets showing significant differential expression. A Benjamini and Hochberg algorithm was implemented in Genespring to reduce the false discovery rate.

Repositories


GEO

GSE11683

ArrayExpress

E-GEOD-11683

BioProject

PRJNA122645

Download


Contact


Ann Chambers