|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||May 26 2009|
|Last update date:||Aug 28 2018|
|Diseases:||Breast Neoplasms, Neoplasms|
|Dataset link||Expression analysis in a breast cancer metastasis model|
For each cell line, 3 biological replicates were grown, RNA isolated, and gene expression profiles determined using Affymetrix HGU133_Plus_2 genechips. The MDA-MB-468GFP-LN cell line was the "experimental" group, and the MDA-MB-468GFP was the control group. Intensity values were calculated using MAS5.0, Data was then imported into Genespring 7.0, and ANOVA (nominal alpha set to 0.05) was used to determine probe sets showing significant differential expression. A Benjamini and Hochberg algorithm was implemented in Genespring to reduce the false discovery rate.