Computational protocol: Late-onset hypersensitivity after a lesion in the ventral posterolateral nucleus of the thalamus: A macaque model of central post-stroke pain

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Protocol publication

[…] To evaluate the spatiotemporal changes of a stroke, anatomical MRI scans were performed before, and 1 day, 4 days, 1 week, 2 weeks, 1 month, and 3 months after collagenase injection. The MRI protocols with both T1- and T2-weighted sequences were the same as those described above. The areas that contained edema and hematoma were seen as a region of high and low signal intensities, i.e. hyperintense and hypointense areas, in T2-weighted MR images, respectively, as in previous studies–. The unbiased volumes for both edema and hematoma were calculated by Cavalieri’s principle, using StereoInvestigator imaging software (MBF Bioscience, Williston, VT, USA). In the atlas-based analysis, the T2-weighted MR images of macaques that received a collagenase injection into the right hemisphere were flipped along the mid-sagittal plane, so that the stroke area was located on the left side of the brain. These processed MR images were matched to macaque brain template (voxel size, 0.5 × 0.5 × 0.5 mm) by using SPM12 software ( After individual images were processed as described, the areas with hypointense signals were delineated manually as regions of interest and overlaid onto the template by using MRIcro software ( size and location of the lesion induced by collagenase injection were also evaluated by histological analysis, using the procedure employed in our previous studies, . After the Behavioral experiments were completed, frozen brain sections at 18 μm of thickness were prepared as in our previous studies–. Images of the Nissl-stained sections were photographed under an Olympus BX60 microscope by using a 3CCD color video camera (DV-47d; MBF Bioscience) and then digitized using StereoInvestigator imaging software. The lesioned area was defined as the area of a dense concentration of small cells (5–10 μm in diameter), which presumably incudes both glial cells and blood cells, and unbiased volumes were calculated using the same method as that for MR image analysis. […]

Pipeline specifications

Software tools SPM, MRIcro
Application Magnetic resonance imaging
Organisms Homo sapiens, Macaca fascicularis
Diseases Drug Hypersensitivity, Cerebrovascular Trauma, Stroke