|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||Apr 14 2014|
|Last update date:||Feb 2 2018|
|Diseases:||Hypoprothrombinemias, Multiple Carboxylase Deficiency, Teratoma|
|Dataset link||Loss of Tet enzymes compromises proper differentiation of embryonic stem cells|
To quantify global gene expression in differentiating embryoid bodies (EBs) derived from wild type (WT) and Tet triple knockout (TKO), TKO and WT mouse embryonic stem cells (ESCs) were differentiated in vitro to EBs and cultured for 10 days. RNA was extracted using Qiagen RNeasy kit and subjected to microarray analysis. Global gene expression profile of two technical replicas of WT embryoid bodies (2 samples in total) was compared to two technical replicas of two independent TKO embryoid bodies (4 TKO samples in total).