Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Apr 14 2014
Last update date: Feb 2 2018
Access: Public
Diseases: Hypoprothrombinemias, Multiple Carboxylase Deficiency, Teratoma
Chemicals: 5-Methylcytosine
Dataset link Loss of Tet enzymes compromises proper differentiation of embryonic stem cells

Experimental Protocol


To quantify global gene expression in differentiating embryoid bodies (EBs) derived from wild type (WT) and Tet triple knockout (TKO), TKO and WT mouse embryonic stem cells (ESCs) were differentiated in vitro to EBs and cultured for 10 days. RNA was extracted using Qiagen RNeasy kit and subjected to microarray analysis. Global gene expression profile of two technical replicas of WT embryoid bodies (2 samples in total) was compared to two technical replicas of two independent TKO embryoid bodies (4 TKO samples in total).

Repositories


GEO

GSE55574

ArrayExpress

E-GEOD-55574

BioProject

PRJNA240086

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Contact


Meelad Dawlaty