|Number of samples:||10|
|Release date:||Jul 15 2018|
|Last update date:||Oct 14 2018|
|Dataset link||High-Resolution Dissection of Conducive Somatic Cell Reprogramming to Naïve Ground State Pluripotency in Mbd3/NuRD and Gatad2a/NuRD Depleted Systems [RRBS]|
Mbd3f/- MEFs that carry DOX-inducible OKSM cassette were used, and iPSC reprogramming was initiated by addition of DOX in FBS/LIF medium in 5% pO2 conditions. On day 3.5 after DOX initiation, medium was changed to LIF/KSR-based with the addition of two small molecule inhibitors for MEK/ERK and GSK3 signaling (2i). Cells were harvested every 24 hours until day 8, and processed for library preparation followed by high-throughput sequencing. Mbd3f/- established iPSC line (after 3 passages or more), and WT v6.5 mouse ESCs were used as positive controls. DNA methylation was measured in each time point using reduced representation bisulfite sequencing (RRBS).