Dataset features

Specifications


Application: BS-seq analysis
Number of samples: 11
Release date: Aug 11 2017
Last update date: Nov 27 2018
Access: Public
Diseases: Neurodegenerative Diseases
Chemicals: Cytosine
Computational protocol: I-TASSER, PyMOL
Dataset link Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein (RRBS)

Experimental Protocol


I) ChIP-seq of plasmid electroporated K562 cells using Illumina Nextseq sequencing system. II) Reduced representation bisulfited sequencing (RRBS) of plasmid transfected HEK 293T cells using Illumina Hiseq2000 sequencing system. III) PCR amplicon deep sequencing of HEK 293T cells, K562 cells or mice tissue using Illumina Nextseq sequencing system.

Repositories


GEO

GSE90690

ENA

SRP094390

BioProject

PRJNA355621

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Contact


Jianzhong Su

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