Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 46
Release date: Sep 8 2009
Last update date: Jan 15 2017
Access: Public
Chemicals: Glucose
Computational protocol: SBEAMS, TM4, Gaggle, BioProspector, MEME, WebLogo
Dataset link Halobacterium NRC-1 VNG1451C deletion mutant growth in defined medium with and without glucose

Experimental Protocol


Halobacterium salinarum NRC-1 (ATCC700922) ura3 parent and VNG1451C strains were grown in complete defined medium (CDM; 20 amino acids at concentrations defined by Shand and Perez, 1999. NaCl 250g/L, MgSO4 20 g/L, KCl 1 g/L, NaH2PO4 0.167mM, Biotin 0.02mM, Thiamin 0.015mM, Folic acid 0.0113 mM, MnSO4 0.01mM, FeSO4, 0.01mM, glucose added at 10% w/v where indicated in sample files) at 37ºC under full-spectrum white light. Biological replicate samples were removed throughout the growth curve at early log, mid log, and stationary phase to measure genome-wide transcription.

Repositories


GEO

GSE13529

ArrayExpress

E-GEOD-13529

BioProject

PRJNA110143

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Contact


Amy Schmid
Amy K Schmid

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