Computational protocol: Analysis of polymorphisms in the circumsporozoite protein gene of Plasmodium vivax isolates from Henan Province, China

Similar protocols

Protocol publication

[…] The nested PCR products of csp genes were electrophoresed on a 1.5% agarose gel. Then directly sequenced in both directions using an ABI PRISM3730 DNA sequencer (Sangon Biotech Co., Ltd, China). The sequences were edited and aligned with the programs Chromsa Pro and BioEdit, phylogenetic trees were made using the Molecular Evolutionary Genetics Analysis (MEGA) 7.0. The reliability of the phylogenetic trees was tested by bootstrapping analysis using 500 replicates. Branches corresponding to partitions reproduced in less than 50% bootstrap replicates are collapsed. The nucleotide sequence data described here were submitted to the GENBANK database under the accession numbers KP888994–KP889001. […]

Pipeline specifications

Software tools BioEdit, MEGA
Application Phylogenetics
Organisms Plasmodium vivax, Toxoplasma gondii