Dataset features

Specifications


Application: DNA methylation array analysis
Number of samples: 121
Release date: May 19 2015
Last update date: Jul 25 2018
Access: Public
Diseases: Anemia, Refractory, Ovarian Neoplasms, Tumor Lysis Syndrome
Chemicals: Platinum
Dataset link Whole genome characterisation of chemoresistant ovarian cancer [Illumina_450K_Methylation]

Experimental Protocol


Genomic DNA (500ng) was bisulfite converted using EZ DNA methylation Kit (Zimo Research) following the manufacturer’s protocol with modification for Illumina Infinium Methyaltion arrays. Samples were hybridized to Infinium Human Methylation 450K BeadChips (Illumina) according to the manufacturer’s protocol. Arrays were scanned on Iscan (Illumina). Data was background corrected, normalized to internal controls and QC was performed at the probe and sample level. COMBAT was used to remove batch effects (Johnson et al., 2007). Contributor: The Australian Ovarian Cancer Study Group

Repositories


GEO

GSE65820

ArrayExpress

E-GEOD-65820

BioProject

PRJNA275137

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Contact


Ann-Marie Patch