Computational protocol: Detection of a Single Identical Cytomegalovirus (CMV) Strain in Recently Seroconverted Young Women

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Protocol publication

[…] Purified PCR products were sequenced using the BigDye Terminator Cycle Sequencing Kit (Perkin-Elmer Applied Biosystems, Foster City, CA). Sequencing products were analyzed with an ABI 3100 automated sequencer (Applied Biosystems). Nucleotide and amino acid sequences of clinical CMV strains and reference Towne, Toledo and AD169 strains were aligned using ClustalW and MAFFT . Phylogenetic trees were constructed using the neighbor- joining method in Mega4 . […]

Pipeline specifications

Software tools Clustal W, MAFFT, MEGA
Application Phylogenetics
Organisms Homo sapiens
Diseases Cytomegalovirus Infections