Computational protocol: Annexin5 Plays a Vital Role in Arabidopsis Pollen Development via Ca2+-Dependent Membrane Trafficking

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Protocol publication

[…] Multiple sequence alignment of the deduced amino acid sequences of repeats I and IV from Arabidopsis AnnAt1 to 8 was performed using DNAMAN (Lynnon Biosoft). A homology model of Ann5 was produced by the Swiss-Model program (http://www.expasy.ch/) and then colored using PyMOL software. The model was based on template 2zocA (2.00 A), and the QMEAN Z-Score was −1.018. […]

Pipeline specifications

Software tools DNAMAN, PyMOL, QMEAN
Application Protein structure analysis
Organisms Arabidopsis thaliana
Chemicals Calcium, Brefeldin A