|Application:||Gene expression microarray analysis|
|Number of samples:||12|
|Release date:||Mar 18 2010|
|Last update date:||Aug 28 2018|
|Genes:||FCP1, CD4, PTPRC, CD44, SELL, SELPLG, SELP, Tsga8, STIM|
|Dataset link||Expression data from effector CD4 T cells isolated from MRL/Faslpr mice.|
Cells were isolated from the spleens of aged (16-24 weeks) female MRL/Faslpr mice directy ex vivo, and immediatley sorted into 3 populations; CD62Lhi PSGL1hi, CD62Llo PSGL1hi, and CD62Llo PSGL1lo. All 3 populations of cells were previously gated on TCRb+, CD4+, B220-, CD44+. Naive CD4 T cells were isolated directly ex vivo from the spleens of young (6-8 weeks) female MRL/Faslpr mice, and immediately sorted by gating on TCRb+, CD4+, B220-, CD62Lhi, CD44lo. Three indepedent sorts were performed. RNA was isolated using Qiagen's RNAeasy kit and total RNA was submitted to the W.M. Keck Foundation Biotechnology Resource Laboratory at Yale for amplification and hybridization to Affymetrix Mouse Genome 430 2.0 GeneChips.