Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Mar 18 2010
Last update date: Aug 28 2018
Access: Public
Diseases: Skin Diseases
Genes: FCP1, CD4, PTPRC, CD44, SELL, SELPLG, SELP, Tsga8, STIM
Dataset link Expression data from effector CD4 T cells isolated from MRL/Faslpr mice.

Experimental Protocol


Cells were isolated from the spleens of aged (16-24 weeks) female MRL/Faslpr mice directy ex vivo, and immediatley sorted into 3 populations; CD62Lhi PSGL1hi, CD62Llo PSGL1hi, and CD62Llo PSGL1lo. All 3 populations of cells were previously gated on TCRb+, CD4+, B220-, CD44+. Naive CD4 T cells were isolated directly ex vivo from the spleens of young (6-8 weeks) female MRL/Faslpr mice, and immediately sorted by gating on TCRb+, CD4+, B220-, CD62Lhi, CD44lo. Three indepedent sorts were performed. RNA was isolated using Qiagen's RNAeasy kit and total RNA was submitted to the W.M. Keck Foundation Biotechnology Resource Laboratory at Yale for amplification and hybridization to Affymetrix Mouse Genome 430 2.0 GeneChips.

Repositories


GEO

GSE20735

ArrayExpress

E-GEOD-20735

BioProject

PRJNA124971

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Contact


Amanda Poholek