Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Mar 2 2013
Last update date: Nov 9 2017
Access: Public
Diseases: Melanoma, Experimental, Neoplasms, Scleroderma, Systemic, Superior Mesenteric Artery Syndrome, Leukemia, T-Cell
Dataset link Studies in a murine B16 melanoma model show that persistent antigen at vaccination sites induces CD8+ T cell sequestration, dysfunction and deletion

Experimental Protocol


To study the fate of melanoma-specific CD8+ T cells after peptide vaccination, we tracked T cell receptor-transgenic pmel-1 T cells in mice vaccinated with heteroclitic gp100_25-33 peptide emulsified in IFA. Splenic pmel-1 CD8+ T cells were purified at 6 and 21 days after vaccination with either gp100/IFA/covax or gp100/saline/covax, and then their total RNA was extracted and used for comparison by gene expression profiling.

Repositories


GEO

GSE43929

ArrayExpress

E-GEOD-43929

BioProject

PRJNA188175

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