|Application:||RNA-seq analysis, sRNA-seq analysis|
|Number of samples:||3|
|Release date:||Apr 30 2011|
|Last update date:||Jun 11 2013|
|Taxon:||Capsicum annuum, Zea mays|
|Dataset link||High-throughput sequencing of small RNAs in Capsicum annuum|
Small RNA libraries were derived from leaves, flowers and fruit of Capsicum annuum. Total RNA was isolated using the TriReagent (Molecular Research Center) for leaves and flowers and the Plant RNA Purification Reagent (Invitrogen) for fruit, and submitted to Illumina (Hayward, CA, http://www.illumina.com) for small RNA library construction using approaches described in (Lu et al., 2007) with minor modifications. The small RNA libraries were sequenced with the Sequencing-By-Synthesis (SBS) technology by Illumina. PERL scripts were designed to remove the adapter sequences and determine the abundance of each distinct small RNA. We thank Barbara Baker for providing the plant material, as well as Kan Nobuta and Gayathri Mahalingam for assistance with the computational methods.
Emanuele De Paoli