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Pipeline publication

[…] ost-specific toxin and a virulence factor. Homologs were found in five fungal species including three in Colletotrichum. While identifying at least one HGT event (ClArg encoding argininosuccinate lyase) from bacteria into the genus Colletotrichum, comparative genomics analysis revealed that ClToxB is a native gene and not horizontally transferred from other species., In a previous study, we constructed a directional cDNA plasmid library from Eston (no resistance to anthracnose) leaf tissues infected with C. lentis isolate CT-21 (race 1) undergoing the morphogenetic biotrophy-necrotrophy transition []. In the present study, 2000 previously uncharacterized ESTs were sequenced and subjected to BLASTX analysis, and 780 ESTs were identified as ESTs of fungal origin. The remaining ESTs either were of plant origin (~53 %) or unknown (~4 %). The ORF finder, and SignalP and iPSORT algorithms were employed to identify open reading frames (ORFs) containing putative signal peptide (SP) sequences. Twenty-two ORFs were predicted to encode proteins with a putative N-terminal SP for secretion. Out of these 22 ESTs, 13 ESTs represented unique sequences (unigenes) and the remaining 9 ESTs were assembled into two individual unique sequences (Table ) and were deposited in the NCBI GenBank EST database (dbEST). The average GC content of these unigenes was close to 59 %.Table 1These effectors were identified from a directional cDNA plasmid library from leaf tissues of Lens culinaris cv. Eston infected with C. lentis isolate CT-21 undergoing the morphogenetic biotrophy-necro […]

Pipeline specifications

Software tools TBLASTX, SignalP, TMHMM