Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 13
Release date: Jul 12 2006
Last update date: Aug 23 2018
Access: Public
Genes: CD8A, GZMA, IL7R, KLRG1
Dataset link Immunodomination leads to selective expansion of the fittest CD8 T cells

Experimental Protocol


B10.H7b female mice were primed by i.p. injection of a cell mixture containing 2 x 107 B10 male splenocytes and 2 x 107 B10.H7b male splenocytes. On day 14 after priming, splenocytes were labeled with anti-CD8 Ab as well as H7a- and HY-Tet labeled with different fluorochromes. Then, three populations of splenocytes were purified using a FACS cell sorting: HYTet+ (n=4), H7aTet+ (n=4), and Tet- (n=5) CD8 T cells. RNA of sorted T cells was extracted and linearly amplified, cRNA was prepared, and Affymetrix Mouse Genome 430 2.0 oligonucleotide arrays were used to analyze gene expression.

Repositories


GEO

GSE2924

ArrayExpress

E-GEOD-2924

BioProject

PRJNA92615

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