|Application:||Gene expression microarray analysis|
|Number of samples:||13|
|Release date:||Jul 12 2006|
|Last update date:||Aug 23 2018|
|Genes:||CD8A, GZMA, IL7R, KLRG1|
|Dataset link||Immunodomination leads to selective expansion of the fittest CD8 T cells|
B10.H7b female mice were primed by i.p. injection of a cell mixture containing 2 x 107 B10 male splenocytes and 2 x 107 B10.H7b male splenocytes. On day 14 after priming, splenocytes were labeled with anti-CD8 Ab as well as H7a- and HY-Tet labeled with different fluorochromes. Then, three populations of splenocytes were purified using a FACS cell sorting: HYTet+ (n=4), H7aTet+ (n=4), and Tet- (n=5) CD8 T cells. RNA of sorted T cells was extracted and linearly amplified, cRNA was prepared, and Affymetrix Mouse Genome 430 2.0 oligonucleotide arrays were used to analyze gene expression.