Computational protocol: First Molecular Detection of Babesia gibsoni in Dogs from Wuhan, China

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Protocol publication

[…] The obtained 18S rRNA and ITS sequences were subjected to BLAST analysis in the GenBank database. Multiple sequence alignment with related genes was conducted using MAFFT (version 7) (Katoh and Frith, ), and the alignment was edited with BioEdit (version 7.0.9). Phylogenetic trees based on the 18S rRNA and ITS nucleotide sequences were constructed using MEGA6 software (Tamura et al., ). All analyses were performed with 1,000 bootstrap replications. […]

Pipeline specifications

Software tools MAFFT, BioEdit, MEGA
Application Phylogenetics
Organisms Canis lupus familiaris
Diseases Babesiosis, Infection