Computational protocol: Early and Degressive Putamen Atrophy in Multiple Sclerosis

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Protocol publication

[…] All 3D MPRAGE images were corrected for contrast and intensity inhomogeneities to reduce segmentation errors using an in-house software (Eval 3.0). Total WM and GM volume, putamen and intracranial volume (ICV), and volume of hypointense WM lesions in T1w images were obtained from FreeSurfer (Version 5.1; []). For details of this method see [,,,]. Additionally, we calculated the putamen volume from FSL-FIRST (Integrated Registration and Segmentation Tool, Oxford University, Oxford, UK), which is incorporated into the FSL software (Version 5.0; []). For details of this method see []. By the application of these two alternative techniques, we achieved quite similar results by Pearson product-moment correlation (R = 0.92). Due to the congruent results between FSL-FIRST and FreeSurfer, we decided to present only the obtained by FreeSurfer. Volume of hyperintense WM lesions in FLAIR images was obtained from LST (Lesion Segmentation Tool) (Version 1.2.3; []) []. We calculated the absolute putamen volume (APV) (unit: mm³) for all participants as (6)APV=right putamen volume + left putamen volume [mm3]From the APV and the absolute ICV (unit: mm³) we calculated the relative (percentage) putamen volume as (7)RPV=APV/ICV× 100%Disease duration was defined as time between the manifestation of patient’s first symptoms and the date of MRI examination. [...] Data were analyzed using STATISTICA™ version 10 (StatSoft (Europe) GmbH, Hamburg, HH, Germany). By performing the Pearson’s Chi-squared test, we analyzed the gender distribution between patients and healthy controls. Differences of age, ICV, APV, RPV, and ΔRPV% (the percentage deviation of the patients’ putamen volume in relation to HC) between patients and HC were tested by two-sided t-tests. Differences were considered as statistically significant if p ≤ 0.05. By linear univariate regression analyses we calculated correlation coefficients of APV and ICV, of RPV and participant’s age, of ΔRPV and participant’s age, and additionally of ΔRPV% and volume of WM lesions in T1w and FLAIR images. To examine the relation between ΔRPV% and the patient’s disease duration, we used the linear function (8)ΔRPV% = a + b × ″disease duration″ and the logarithmic function (a and b estimated by lest squares fitting) (9)ΔRPV% = a × log10(″disease duration″)+ b […]

Pipeline specifications

Software tools FreeSurfer, Statistica
Applications Miscellaneous, Magnetic resonance imaging
Organisms Homo sapiens
Diseases Multiple Sclerosis, Multiple Sclerosis, Relapsing-Remitting, Acute Kidney Injury