Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 24
Release date: Dec 30 2006
Last update date: Jul 1 2016
Access: Public
Diseases: Autoimmune Diseases, Polyneuropathies
Dataset link Impairment of organ-specific T cell negative selection by diabetes susceptibility genes: analysis by mRNA profiling

Experimental Protocol


Analysis of two thymic populations - early single positive thymocytes (CD4+CD8lowCD69+TCRhi) and early double positive thymocytes (CD4+CD8+CD69-TCRlow). Samples purified from the 3A9 TCR transgenic (TCR) to characterise positive selection and the 3A9 TCR x insHEL double transgenic (Dbl) to characterise negative selection towards an Aire-dependent organ-specific antigen. Both the non autoimmune C57BL10.H2k (B10) and autoimmune Non obese diabetic (NOD) strains were analysed in this method. Three biological replicates were used for each of these eight different groups.

Repositories


GEO

GSE3997

ArrayExpress

E-GEOD-3997

BioProject

PRJNA94273

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Contact


Kristine Hardy

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