Computational protocol: Human longevity is characterised by high thyroid stimulating hormone secretion without altered energy metabolism

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Protocol publication

[…] Descriptive statistics were used to summarise the characteristics of both study groups. Chi square test and t-test were used to describe differences between offspring and partners regarding sex, age, age of the parents, body composition, medical history, medication, creatinine clearance, liver function tests, smoking and alcohol use. To calculate differences in secretion between offspring and partner for the different thyroid status parameters, areas under the curves were calculated according to the trapezoid method using SigmaPlot for Windows Version 11.0 (Systat Software, GmbH, Erkrath, Germany). The areas under the curves were calculated over the 24 hours, during the day from 09.00 h to 23.00 h and during the night from 24.00 h to 06.00 h. Linear regression was used to compare total TSH secretion, levels of TH, T3, rT3, measurements of AUCs, levels of cAMP/TSH ratio, AUCfT4/total TSH secretion, fT4/TSH ratio, fT4xTSH product, fT3/fT4 ratio, T3/rT3 ratio, AUC fT3/AUC fT4 ratio and parameters of the energy metabolism between offspring and partners adjusted for age and sex. When not normally distributed, parameters were log transformed for analysis, and data are presented as geometric mean with 95% confidence interval. Because ApEn of TSH was still not normally distributed after log transformation, a non-parametric test was used. For all above mentioned analyses the Statistical Package for the Social Sciences program for Windows, version 20 (SPSS, Chicago, IL) was used. Graphs were made using GraphPad Prism version 5 (GraphPad, San Diego, CA). […]

Pipeline specifications

Software tools SigmaPlot, SPSS
Application Miscellaneous
Organisms Homo sapiens
Diseases Hypothalamic Neoplasms