Computational protocol: Malignant ascites enhances migratory and invasive properties of ovarian cancer cells with membrane bound IL-6R in vitro

Similar protocols

Protocol publication

[…] Cells were full plated with complete media in 6-well plates and incubated at 37°C for 24 hr. The complete medium was changed to either fresh complete media or 10% of the indicated ascites. The cell layer was scratched with a pipette tip to create an artificial wound. The wound closed area (in arbitrary unit) was measured using ImageJ software. [...] Data were presented as mean ± SD of triplicate experiments. One-way ANOVA and, when appropriate, Student's t-test were used for statistical analyses. Significant difference among experimental groups was analyzed by Scheffe's post hoc test. All analyses were conducted using IBM SPSS statistics 21 (SPSS Inc., Chicago, IL). P values of < 0.05 were considered statistically significant. […]

Pipeline specifications

Software tools ImageJ, SPSS
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Homo sapiens