Dataset features

Specifications


Application: SNP array data analysis
Number of samples: 169
Release date: Nov 15 2017
Last update date: Aug 12 2018
Access: Public
Dataset link Effects of oxidative stress on mitotic recombination and genomic stability in yeast

Experimental Protocol


JSC25 cells were grown in 7 mL YPD overnight to reach an OD600 of 0.2. Yeast cells (5×107) were collected by centrifugation and arrested in G1 in 1 mL YPD containing 2.5 μg α-factor at 300. After two hours, H2O2 was added into the cell culture. Cell culture was incubated at 300 for another hour. Yeast cells were then washed twice to remove α-factor and H2O2 before plating. Yeast cells were cultured at 300 for 3 days and sectored colonies were selected for whole genome SNP microarray analysis.

Repositories


GEO

GSE106816

BioProject

PRJNA418280

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Contact


Daoqiong Zheng