Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Jun 12 2013
Last update date: Jul 26 2018
Access: Public
Genes: LMO3
Taxon: Homo sapiens
Dataset link Expression data from mock- or LMO3 silenced differentiating adipose stem cells

Experimental Protocol


Human preadipocytes (human adipose stem cells) were obtained from lipoaspirates by enzamytic digestions, followed by several steps of centrifugations (Mikkelsen et. al Cell. 2010 Oct 1;143(1):156-69.). Following isolation, human adipose stem cells were transfected with a control siRNA (siCtrl) or two different siRNA oligos targeting the gene LMO3 (siLMO3_oligo1 or siLMO3_oligo2). 40Hrs later, the transfected human adipose stem cells were induced to differentiate into mature adipocytes with a adipogenic cocktail (FullMix) and RNA isolated at day 0 or day6 after induction of differentiation.

Repositories


GEO

GSE41712

ArrayExpress

E-GEOD-41712

BioProject

PRJNA178009

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Contact


Markus Jeitler

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