Dataset features

Specifications


Dataset type: Non-coding RNA profiling by high throughput sequencing
Number of samples: 12
Release date: Dec 14 2018
Last update date: Feb 14 2019
Access: Public
Dataset link Knockdown of heterochromatin protein 1 binding protein 3 recapitulates phenotypic, cellular, and molecular features of aging [miRNA-Seq]

Experimental Protocol


Viral vectors encoding either shRNA for Hp1bp3 (AAV9-shRNA-Hp1bp3) or a scrambled control (AAV-scrmb-shRNA) were delivered bilaterally into the hippocampus of adult (3-5 month old) C57BL/6J and DBA/2J mice. Three male mice from each group were used for sequencing. Briefly, each mouse was anesthetized using isoflurane, brains were quickly removed, and the hippocampus immediately dissected and snap-frozen in liquid nitrogen. Samples were kept at -80 until further use. RNA quality was evaluated using a BioAnalyzer, libraries prepared, and sequenced on a Illumina HiSeq 2500. A pairs of miRNA samples given identical barcodes during the initial sequencing. All samples were re-sequenced and where available, fastq files from both runs were concatenated, providing expanded sequencing depth for 10/12 samples.

Repositories


GEO

GSE119319

ENA

SRP159464

BioProject

PRJNA489080

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Contact


Catherine Kaczorowski