Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Jul 1 2011
Last update date: Mar 30 2012
Access: Public
Diseases: Infection
Dataset link Liver-expressed antimicrobial peptide-2 is downregulated in Eimeria maxima-infected chickens

Experimental Protocol


Total RNA was extracted from the jejunum of control and challenged chicks from both lines A and B. Microarrays were used for detecting the expression-changed genes which responded to the Eimeria challenge. Samples included: four control A chicks, two challenged A chicks with a lesion score of 1 (A/LS1), two challenged A chicks with lesion scores of 3 to 4 (A/LS3-4), four control B chicks, two challenged B chicks with lesion scores of 2 to 3 (B/LS2-3) and two challenged B chicks with lesion scores of 4 (B/LS4). The DNA microarrays were processed at the Virginia Bioinformatics Institute (Virginia Tech) core facility. The raw array data were normalized using GC-robust multiple array (GC-RMA) normalization. Analysis of variance was used for differentially expressed genes based on Welch ANOVA (P < 0.05) and probe set lists were ordered using the fold change analysis provided by GeneSpring software.

Repositories


GEO

GSE25588

ArrayExpress

E-GEOD-25588

BioProject

PRJNA134225

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Contact


Huifeng Li