Computational protocol: Ectodomain Movements of an ATP-gated Ion Channel (P2X2 Receptor) Probed by Disulfide Locking*

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Protocol publication

[…] Homology models of the rat P2X2 receptor were made with MODELLER () (version 9.10) based on the closed and open zebrafish P2X4.1 crystal structures (Protein Data Bank codes 4DW0 and 4DW1). The lowest energy models were assessed with MolProbity () and minimized using AMBER (). Models had 98.9% (closed) and 97.4% (open) residues in the allowed regions of the Ramachandran plot. Images were made in Chimera (version 1.62; ). […]

Pipeline specifications

Software tools MODELLER, MolProbity, AMBER
Application Protein structure analysis
Organisms Rattus norvegicus, Homo sapiens
Chemicals Adenosine Triphosphate, Dithiothreitol