|Number of samples:||1324|
|Release date:||Aug 11 2018|
|Last update date:||Nov 23 2018|
|Taxon:||Homo sapiens, Danio rerio|
|Dataset link||Single-cell RNA sequencing of zebrafish beta-cells from various stages|
We used fluorescence-activated cell sorting (FACS) coupled with next generation RNA-Sequencing to profile beta-cells from various stages. Cells were sorted into a 96-well plates and single-cell library prepared using SMART-Seq v4 Ultra Low Input RNA Kit . Sequencing was performed on llumina Nextseq500 aiming at an average sequencing depth of 0.5 million reads per cell. Reads were splice-aligned to the zebrafish genome, GRCz10, using HISAT2. htseq-count was used to assign reads to exons thus eventually getting counts per gene.