Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Oct 25 2013
Last update date: Aug 3 2015
Access: Public
Diseases: Bacterial Infections
Chemicals: Ecdysone, Steroids
Dataset link Gene expression in Drosophila hemocytes at the onset of metamorphosis, and dependence to the Ecdysone Receptor

Experimental Protocol


Hemocytes were isolated by FACS from selected 3rd instar larvae (at the late feeding stage) and prepupae (from 1h to 2h after puparium formation - APF) corresponding to two different genotypes: individuals w;HmlDeltaGal4, UAS-GFP/+ that express GFP specifically in hemocytes (genotype control), and individuals w;HmlDeltaGal4; UAS-GFP/UAS-EcRB1DN W650A which hemocytes express an Ecdysone Receptor Dominant Negative construct in addition to the GFP (EcRDN). For each of the four conditions we performed three biological replicates.

Repositories


GEO

GSE49326

ArrayExpress

E-GEOD-49326

BioProject

PRJNA213633

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