Computational protocol: Polysaccharide biosynthesis-related genes explain phenotype-genotype correlation of Microcystis colonies in Meiliang Bay of Lake Taihu, China

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Protocol publication

[…] Alignment for all of the sequences was determined by Muscle and edited by software Bioedit. Some related sequences in the NCBI database were also used for alignment. MEGA5 was used to construct neighbor-joining tree of phylogeny analysis, with bootstrap for 1000 replications, Maximum Composite Likelihood, and d: Transitions + Transversions. […]

Pipeline specifications

Software tools MUSCLE, BioEdit, MEGA
Applications Phylogenetics, Nucleotide sequence alignment
Organisms Hemisus marmoratus