Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Apr 1 2013
Last update date: Jul 2 2013
Access: Public
Diseases: Bone Marrow Diseases, Hypoxia, Brain, Multiple Myeloma, Neoplasms
Dataset link Hypoxia-inducible factor (Hif)-1 knockdown blocks tumor growth, myeloma-induced angiogenesis and bone destruction in vivo

Experimental Protocol


The transcriptional profiles on JJN3 transduced with shRNA anti-HIF-1 (JJN3-anti-HIF-1), as compared to those infected with the control vector pLKO.1 (JJN3-pLKO.1), have been analyzed either in hypoxic or normoxic conditions. To perform gene expression profiles, total RNA was purified using the RNeasy Total RNA Isolation Kit (Qiagen, Valencia, CA). Preparation of biotin-labeled cRNA, hybridization to GeneChip Human Genome U133 Plus 2.0 Arrays and scanning (GeneChip¨ Scanner 3000 7G, Affymetrix Inc.) were performed according to manufacturer's protocols.

Repositories


GEO

GSE40326

ArrayExpress

E-GEOD-40326

BioProject

PRJNA173542

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Contact


Luca Agnelli