Computational protocol: Galectin-8 as an immunosuppressor in experimental autoimmune encephalomyelitis and a target of human early prognostic antibodies in multiple sclerosis

Similar protocols

Protocol publication

[…] Western blot analysis was performed using recombinant Gal-8 to screen sera of patients with MS for anti-Gal-8 antibodies, as previously described in patients with SLE or AR []. The same positive and negative controls were included in each western blot and densitometric analysis of the bands was performed by ImageJ. Bands that duplicate the intensity of the negative control in at least two independent experiments performed in triplicate and blinded with respect to the sample were considered positive for anti-Gal-8 reactivity. [...] The presence of anti-Gal-8 autoantibodies in RRMS, PPMS and SPMS patients was analyzed respect to various clinical, radiological and CSF variables using Mann-Whitney U test (with Tukey multiple comparisons) and SPSS Statistics 19.0 software. Yates chi-square corrected test and Odds ratio were calculated with VassarStats. EAE disease scores were analyzed with Mann–Whitney U test or one-way ANOVA Kruskal Wallis with Dunn post-test using Prism 5 software (GraphPad Software). Other analyses were made with two tailed non-paired Student’s t-test as indicated in figure legends. […]

Pipeline specifications

Software tools ImageJ, SPSS, VassarStats
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Homo sapiens, Mus musculus
Diseases Autoimmune Diseases, Encephalomyelitis, Movement Disorders, Multiple Sclerosis, Demyelinating Autoimmune Diseases, CNS