Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: May 22 2012
Last update date: Oct 29 2018
Access: Public
Diseases: Bone Diseases, Multiple Myeloma
Dataset link In vitro and in vivo evidences of osteocytes involvement in myeloma-induced osteoclast formation and bone disease

Experimental Protocol


This series of microarray experiments contains the gene expression profiles of HOB-01 osteocytes cultured alone and co-cultured with the JJN3 human myeloma cell line. 5 micrograms of total RNA was processed, and in accordance with the manufacturer's protocols, the fragmented biotin-labelled cRNA were hybridized on GeneChip Human Genome U133 Plus 2.0 Arrays (Affymetrix Inc.). The arrays were scanned using the Agilent GeneChip Scanner 3000 7G. The images were acquired using Affymetrix GeneChip Operating System (GCOS) 1.1 software and the probe level data converted to expression values using default GCOS1.1 scaling. Natural-scaled data are provided.

Repositories


GEO

GSE27372

ArrayExpress

E-GEOD-27372

BioProject

PRJNA137275

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Contact


Luca Agnelli
Antonino Neri