|Application:||Gene expression microarray analysis|
|Number of samples:||20|
|Release date:||Jan 1 2016|
|Last update date:||Aug 13 2018|
|Diseases:||Hepatitis B, Carcinoma, Hepatocellular, Liver Diseases|
|Dataset link||Gene expression response to HBV infection in PHH|
Primary human hepatocytes (PHH) were prepared from adult patients undergoing lobectomy or segmental liver resection. HBV was concentrated from the supernatant of HepG2.2.15 cells using centrifugal filter devices and tittered by HBV-DNA dot blot analysis after sedimentation into a CsCl density-gradient to determine enveloped DNA-containing viral particles. PHH were isolated from surgical liver resections, cultured, and infected with HBV. Infected PHH and corresponding controls were kept for 4 hours, 8 hours, and 1, 6, and 12 days. Supernatants were obtained to validate the efficiency of infection by ELISA and qRT-PCR, and nucleic acids were extracted for expression and DNA methylation analyses.