Computational protocol: The Effects of Old Age on Hepatic Stellate Cells

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Protocol publication

[…] Two blocks of glutaraldehyde-fixed liver tissue from each animal were cut and studied using a Philips CM10 transmission electron microscope. Approximately 20 HSC for each animal were photographed at 2,600 magnification and subsequently analyzed using ImageJ (http://rsbweb.nih.gov/ij/). The larger diameter of each lipid droplet contained in the cell cytoplasm and the area of each cell was determined. The number of lipid droplets per HSC was also analysed only in those sections where the HSC contained a nucleus in an attempt to compensate for the possibility that the sections were taken in different planes. In total we analyzed 680 droplets and 108 HSC from young rats, 806 droplets and 98 HSC from old rats, 170 droplets and 59 HSC from young mice, and 351 droplets and 57 HSC from old mice. [...] The results are expressed as average ± standard deviation and comparisons performed using parametric and nonparametric, two-tailed Students t-test, depending upon the Schapiro Wilk test for normality (SigmaPlot v11). […]

Pipeline specifications

Software tools ImageJ, SigmaPlot
Applications Miscellaneous, cryo-EM, Microscopic phenotype analysis
Organisms Rattus norvegicus, Mus musculus