Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 14
Release date: Aug 23 2018
Last update date: Nov 30 2018
Access: Public
Chemicals: Morphine
Taxon: Mus musculus
Dataset link Single Cell RNAseq Uncovers a Robust Transcriptional Response to Morphine by Glia

Experimental Protocol


For single-cell experiments, we perfoed Drop-seq on 4 biological replicates each of the nucleus accumbens from saline- or morphine-treated mice. For bulk RNAseq experiments, we performed FACS to purify oligodendrocytes from the entire brains of 6 mice (3 mock, 3 morphine), then isolated RNA and subjected to RNA sequencing.

Repositories


GEO

GSE118918

ENA

SRP158604

BioProject

PRJNA487317

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Contact


Robi Mitra