Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Aug 1 2008
Last update date: Mar 17 2012
Access: Public
Chemicals: Mannose
Dataset link Pig SISP jejunal segments: Lactobacillus plantarum and msa/srt mutant strains vs control, perfusion 4 or 8 hours

Experimental Protocol


A Small Intestinal Segment Perfusion (SISP) test was performed using 4 pigs. 10 segments were prepared in the jejunum of each pig and perfused with Lactobacillus plantarum 299v wildtype, Lactobacillus plantarum 299v msa mutant strain, Lactobacillus plantarum 299v srtA mutant strain, Salmonella typhimurium or PBS (control) for 4 or 8 hours. Pooled samples from each treatment at each timepoint were used for microarray analysis. 8 comparisons were done: L. plantarum wildtype vs control (4 hours), L. plantarum wildtype vs control (8 hours), L. plantarum msa mutant vs control (4 hours), L. plantarum msa mutant vs control (8 hours), L. plantarum srt mutant vs control (4 hours), L. plantarum srt mutant vs control (8 hours), S. typhimurium vs control (8 hours), samples taken at the beginning of the experiment vs control (8 hours). Dye-swaps were performed for each comparison.

Repositories


GEO

GSE9209

ArrayExpress

E-GEOD-9209

BioProject

PRJNA102793

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Contact


Gabriele Gross