Dataset features

Specifications


Application: SPIM
Number of samples: 8
Release date: May 22 2018
Last update date: Nov 19 2018
Access: Public
Taxon: Homo sapiens
Dataset link CRISPR/Cas9-mediated knock-in of an optimized TetO repeat for live cell imaging does not cause heterochromatinization of endogenous loci

Experimental Protocol


We performed CUT&RUN using an antibody specific for H3K9me3 to identify H3K9me3 enriched regions in the genome. We controlled H3K9me3 enrichment flanking one of the target sites we named as ‘region 3’ (gRNA target site is chr6:31,813,898-31,813,920 (hg19)), where we integrated TetO repeats by CRISPR/Cas9-mediated knock-in. We compared clones with 96-mer or 48-mer TetO integration at ‘region 3’ (clones 96-8 and 48-15, respectively) versus wild-type cells. We used non-transduced wild-type cells (wt) and TetR-EGFP transduced wild-type (wtGFP) as controls. The experimental samples analyzed were 96-8 and 48-15 clones transduced with TetR-EGFP. Experiments were performed in duplicates (8 samples total).

Repositories


GEO

GSE113832

ENA

SRP143989

BioProject

PRJNA454207

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Contact


Ipek Tasan